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mascot generic format (mgf) files  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mascot generic format (mgf) files
    Mascot Generic Format (Mgf) Files, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mascot+generic+format+(mgf)+files/mascot+generic+format++mgf++files/pm38583233-69-0-10
    Average 90 stars, based on 1 article reviews
    mascot generic format (mgf) files - by Bioz Stars, 2026-10
    90/100 stars

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    Generated:

    Article Title: Discovery of a small protein-encoding cis -regulatory overlapping gene of the tumor suppressor gene Scribble in humans
    Article Snippet: The raw tryptic mass spectra files were first processed to recalibrate precursor masses and convert the files into Mascot Generic Format (MGF) files, which contain MS/MS peak lists and experimental parameters, with Thermo Proteome Discoverer (Thermo Fisher Scientific).

    Article Title: Comparative Monomethylarginine Proteomics Suggests that Protein Arginine Methyltransferase 1 (PRMT1) is a Significant Contributor to Arginine Monomethylation in Toxoplasma gondii
    Article Snippet: Database Searches Raw mass spectra from LC-MS/MS were converted to Mascot Generic Format (MGF) files using Proteome Discoverer 1.2 (Thermo Fisher Scientific) software and then searched against a combined database (entries = 27608) of Homo sapiens (downloaded from Uniprot.org, Feb 25th, 2015) proteins and Toxoplasma gondii ME49 (downloaded from ToxoDB.org, version 12) proteins using an in-house Mascot search engine (Matrix Science, version 2.5.1, Boston, MA) and the Mascot default decoy database to obtain protein and peptide %FDRs.

    Article Title: Proteomic Profiling of Rhabdomyosarcoma-Derived Exosomes Yield Insights into Their Functional Role in Paracrine Signaling.
    Article Snippet: Subscriber access provided by Nottingham Trent University is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Article Title: Transcriptome and proteome analyses reveal complex mechanisms of reproductive diapause in the two-spotted spider mite, Tetranychus urticae.
    Article Snippet: Although a variety of factors underlying diapause have been identified in arthropods and other organisms, the molecular mechanisms regulating diapause are still largely unknown.. Here, to better understand this process, we examined diapause-associated genes in the two-spotted spider mite, Tetranychus urticae, by comparing the transcriptomes and proteomes of early diapausing and reproductive adult females.. Amongst genes underlying diapause revealed by the transcriptomic and proteomic data sets, we described the noticeable change in Caassociated genes, including 65 Ca-binding protein genes and 23 Ca transporter genes, indicating that Ca signalling has a substantial role in diapause regulation.

    Article Title: The Small RNA Teg41 Regulates Expression of the Alpha Phenol-Soluble Modulins and Is Required for Virulence in Staphylococcus aureus
    Article Snippet: Spectra were extracted from raw data files and converted into Mascot Generic Format (mgf) files using Proteome Discoverer 2.2. (Thermo Fisher) and then analyzed using the search engine Mascot (version 2.6.1; Matrix Science, London, UK).

    Article Title: Online Removal of Sodium Dodecyl Sulfate via Weak Cation Exchange in Liquid Chromatography-Mass Spectrometry Based Proteomics.
    Article Snippet: Raw MS/MS data was converted into Mascot Generic Format (mgf) files using Thermo Proteome Discoverer (version 1.4.1.14, Thermo Fisher Scientific Inc., Bremen, Germany).

    Article Title: Comparative Monomethylarginine Proteomics Suggests that Protein Arginine Methyltransferase 1 (PRMT1) is a Significant Contributor to Arginine Monomethylation in Toxoplasma gondii
    Article Snippet: Database Searches: Raw mass spectra from LC-MS/MS were converted to Mascot Generic Format (MGF) files using Proteome Discoverer 1.2 (Thermo Fisher Scientific) software and then searched against a combined database (entries= 27608) of Homo sapiens (downloaded from Uniprot.org, Feb 25th, 2015) proteins and Toxoplasma gondii ME49 (downloaded from ToxoDB.org, version 12) proteins using an in-house Mascot search engine (Matrix Science, version 2.5.1) and the Mascot default decoy database to obtain protein and peptide %FDRs.

    Article Title: Maackia amurensis seed lectin (MASL) and soluble human podoplanin (shPDPN) sequence analysis and effects on human oral squamous cell carcinoma (OSCC) cell migration and viability.
    Article Snippet: Peak list MASCOT Generic Format (MGF) files were generated by Thermo Proteome Discoverer (v. 2.4) and searched against a database including relevant MASL and variant sequences plus a fasta database composed of common lab contaminants (CRAP) using an in-house installation of GPM Fury (X!Tandem Alanine).

    Variant Assay:

    Article Title: Discovery of a small protein-encoding cis -regulatory overlapping gene of the tumor suppressor gene Scribble in humans
    Article Snippet: The raw tryptic mass spectra files were first processed to recalibrate precursor masses and convert the files into Mascot Generic Format (MGF) files, which contain MS/MS peak lists and experimental parameters, with Thermo Proteome Discoverer (Thermo Fisher Scientific).

    Article Title: Comparative Monomethylarginine Proteomics Suggests that Protein Arginine Methyltransferase 1 (PRMT1) is a Significant Contributor to Arginine Monomethylation in Toxoplasma gondii
    Article Snippet: Database Searches Raw mass spectra from LC-MS/MS were converted to Mascot Generic Format (MGF) files using Proteome Discoverer 1.2 (Thermo Fisher Scientific) software and then searched against a combined database (entries = 27608) of Homo sapiens (downloaded from Uniprot.org, Feb 25th, 2015) proteins and Toxoplasma gondii ME49 (downloaded from ToxoDB.org, version 12) proteins using an in-house Mascot search engine (Matrix Science, version 2.5.1, Boston, MA) and the Mascot default decoy database to obtain protein and peptide %FDRs.

    Article Title: Proteomic Profiling of Rhabdomyosarcoma-Derived Exosomes Yield Insights into Their Functional Role in Paracrine Signaling.
    Article Snippet: Subscriber access provided by Nottingham Trent University is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Article Title: Transcriptome and proteome analyses reveal complex mechanisms of reproductive diapause in the two-spotted spider mite, Tetranychus urticae.
    Article Snippet: Although a variety of factors underlying diapause have been identified in arthropods and other organisms, the molecular mechanisms regulating diapause are still largely unknown.. Here, to better understand this process, we examined diapause-associated genes in the two-spotted spider mite, Tetranychus urticae, by comparing the transcriptomes and proteomes of early diapausing and reproductive adult females.. Amongst genes underlying diapause revealed by the transcriptomic and proteomic data sets, we described the noticeable change in Caassociated genes, including 65 Ca-binding protein genes and 23 Ca transporter genes, indicating that Ca signalling has a substantial role in diapause regulation.

    Article Title: The Small RNA Teg41 Regulates Expression of the Alpha Phenol-Soluble Modulins and Is Required for Virulence in Staphylococcus aureus
    Article Snippet: Spectra were extracted from raw data files and converted into Mascot Generic Format (mgf) files using Proteome Discoverer 2.2. (Thermo Fisher) and then analyzed using the search engine Mascot (version 2.6.1; Matrix Science, London, UK).

    Article Title: Online Removal of Sodium Dodecyl Sulfate via Weak Cation Exchange in Liquid Chromatography-Mass Spectrometry Based Proteomics.
    Article Snippet: Raw MS/MS data was converted into Mascot Generic Format (mgf) files using Thermo Proteome Discoverer (version 1.4.1.14, Thermo Fisher Scientific Inc., Bremen, Germany).

    Article Title: Comparative Monomethylarginine Proteomics Suggests that Protein Arginine Methyltransferase 1 (PRMT1) is a Significant Contributor to Arginine Monomethylation in Toxoplasma gondii
    Article Snippet: Database Searches: Raw mass spectra from LC-MS/MS were converted to Mascot Generic Format (MGF) files using Proteome Discoverer 1.2 (Thermo Fisher Scientific) software and then searched against a combined database (entries= 27608) of Homo sapiens (downloaded from Uniprot.org, Feb 25th, 2015) proteins and Toxoplasma gondii ME49 (downloaded from ToxoDB.org, version 12) proteins using an in-house Mascot search engine (Matrix Science, version 2.5.1) and the Mascot default decoy database to obtain protein and peptide %FDRs.

    Article Title: Maackia amurensis seed lectin (MASL) and soluble human podoplanin (shPDPN) sequence analysis and effects on human oral squamous cell carcinoma (OSCC) cell migration and viability.
    Article Snippet: Peak list MASCOT Generic Format (MGF) files were generated by Thermo Proteome Discoverer (v. 2.4) and searched against a database including relevant MASL and variant sequences plus a fasta database composed of common lab contaminants (CRAP) using an in-house installation of GPM Fury (X!Tandem Alanine).



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